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Sensitivity Enhancement in Transverse Relaxation Optimized NMR Spectroscopy
Author(s) -
Zhu Guang,
Kong Xiangming,
Yan Xianzhong,
Sze Konghong
Publication year - 1998
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19981102)37:20<2859::aid-anie2859>3.0.co;2-4
Subject(s) - nuclear magnetic resonance spectroscopy , relaxation (psychology) , transverse relaxation optimized spectroscopy , spectroscopy , anisotropy , transverse plane , sensitivity (control systems) , biomolecule , nuclear magnetic resonance , dipole , spectrometer , materials science , analytical chemistry (journal) , chemistry , fluorine 19 nmr , chemical physics , physics , optics , nanotechnology , chromatography , electronic engineering , organic chemistry , psychology , social psychology , structural engineering , quantum mechanics , engineering
Dramatically shortened transverse relaxation times in transverse relaxation optimized spectroscopy (TROSY) result from interference between dipole–dipole interactions and the anisotropy of the chemical shift. Thus NMR spectroscopy becomes a suitable method for studying large biomolecules, with optimal performance when 1‐GHz spectrometers become available. By using new phase cycles and data‐processing methods, the sensitivity of the TROSY experiment was increased by a factor of √2, which is of considerable importance for applications in high‐field NMR studies on large proteins.