Premium
Rapid Phosphodiester Hydrolysis by Zirconium( IV )
Author(s) -
Ott Reina,
Krämer Roland
Publication year - 1998
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19980803)37:13/14<1957::aid-anie1957>3.0.co;2-0
Subject(s) - phosphodiester bond , tris , hydroxymethyl , zirconium , chemistry , cerium , hydrolysis , phosphate , ligand (biochemistry) , redox , dna , thymidine , cleavage (geology) , combinatorial chemistry , inorganic chemistry , stereochemistry , organic chemistry , biochemistry , gene , materials science , receptor , rna , fracture (geology) , composite material
A 3×10 9 ‐fold faster cleavage of DNA dinucleotide 1 to thymidine and phosphate can be achieved under mild conditions (pH 5.5, 20°C) with a zirconium( IV ) complex containing tris(hydroxymethyl)aminomethane as ligand [Eq. (1)]. In view of applications of artificial DNAses to molecular biology and gene therapy, non‐redox‐active zirconium( IV ) is an alternative to the highly efficient but reduction‐sensitive cerium( IV ).