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Enantiomeric Enrichment of Stereolabile Chiral Spiro Compounds by Dynamic HPLC on Chiral Stationary Phases
Author(s) -
Lorenz Klaus,
Yashima Eiji,
Okamoto Yoshio
Publication year - 1998
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19980803)37:13/14<1922::aid-anie1922>3.0.co;2-#
Subject(s) - enantiomer , chiral stationary phase , chemistry , chiral column chromatography , chiral derivatizing agent , high performance liquid chromatography , chromatography , stereochemistry
The separation and enrichment of enantiomers can be combined by HPLC on chiral stationary phases. One requirement for success is that the components of the racemate—as, for example, with 1 —undergo thermal interconversion. In the two‐column system shown below the enantiomers are partially separated in the first column at low temperature. The chromatographic flow is then stopped to allow the first fraction eluted (A) to equilibrate on the second column.