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Selectivity of DNA Replication: The Importance of Base‐Pair Geometry over Hydrogen Bonding
Author(s) -
Diederichsen Ulf
Publication year - 1998
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19980703)37:12<1655::aid-anie1655>3.0.co;2-2
Subject(s) - replication (statistics) , thymine , dna replication , base pair , selectivity , hydrogen bond , groove (engineering) , dna polymerase , polymerase , chemistry , nucleotide , geometry , dna , crystallography , stereochemistry , biophysics , biology , materials science , biochemistry , mathematics , molecule , gene , organic chemistry , virology , metallurgy , catalysis
The polymerase enzyme hardly differentiates between thymine and difluorotoluene nucleotides with respect to the replication selectivity. X‐ray structure analysis show the importance of the Watson–Crick geometry for the replication fidelity; base selection is determined by the spatial prerequisite of the substrate pocket and directed protein interactions in the minor groove (schematically shown on the right).