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Lipases: Interfacial Enzymes with Attractive Applications
Author(s) -
Schmid Rolf D.,
Verger Robert
Publication year - 1998
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19980703)37:12<1608::aid-anie1608>3.0.co;2-v
Subject(s) - lipase , transesterification , hydrolysis , enzyme , substrate (aquarium) , chemistry , organic chemistry , serratia marcescens , candida antarctica , triacylglycerol lipase , catalysis , biochemistry , biology , gene , ecology , escherichia coli
Unusually versatile substrate specificity is shown by lipases. Not only do they hydrolyze triacylglycerols—for example, in the stomach and intestine during digestion of dietary fat—and various synthetic esters and amides, but their high stability in organic solvents permits their use in transesterification reactions and ester synthesis as well. Reactions based on lipase catalysis usually proceed with high regio‐ and enantioselectivity. Thus, the Ca 2+ antagonist diltiazem ( 1 ) was obtained with lipase from Serratia marcescens . Over 30 lipases have been cloned in the last few years. Since the tertiary structure of 12 lipases is known, there are presently significant efforts to improve this class of enzymes by protein engineering techniques, in view of their use in detergents and other fields of industrial application.