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Dual Recognition of Double‐Stranded DNA by 2′‐Aminoethoxy‐Modified Oligonucleotides
Author(s) -
Cuenoud Bernard,
Casset Florence,
Hüsken Dieter,
Natt François,
Wolf Romain M.,
Altmann KarlHeinz,
Martin Pierre,
Moser Heinz E.
Publication year - 1998
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19980518)37:9<1288::aid-anie1288>3.0.co;2-u
Subject(s) - oligonucleotide , dna , dual (grammatical number) , computational biology , biology , chemistry , genetics , philosophy , linguistics
Simultaneous interaction of the 2′‐aminoethoxy‐modified oligonucleotides with the phosphodiester backbone (shown on the right, A) and with the bases through Hoogsteen base contacts (B) is seen at each base‐pair step of the duplex DNA target. The electrostatic interaction between the protonated amino group and the negatively charged phosphate group provides for a dramatic increase in the binding affinity and the association rate constant.