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Molecular Beacons: A Novel DNA Probe for Nucleic Acid and Protein Studies
Author(s) -
Tan Weihong,
Fang Xiaohong,
Li Jeffery,
Liu Xiaojing
Publication year - 2000
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/(sici)1521-3765(20000403)6:7<1107::aid-chem1107>3.0.co;2-9
Subject(s) - molecular beacon , nucleic acid , beacon , dna , computational biology , chemistry , biology , genetics , computer science , oligonucleotide , telecommunications
A new concept has been introduced for molecular beacon DNA molecules. Molecular beacons are a new class of oligonucleotides that can report the presence of specific nucleic acids in both homogeneous solutions and at the liquid‐solid interface. They emit an intense fluorescent signal only when hybridized to their target DNA or RNA molecules. Biotinylated molecular beacons have been designed and used for the development of ultrasensitive DNA sensors and for DNA molecular interaction studies at a solid‐liquid interface. Molecular beacons have also been used to study protein‐DNA interactions. They have provided a variety of exciting opportunities in DNA/RNA/protein studies.