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The Effects of Avidin on Inclusion Phenomena and Fluorescent Properties of Biotin‐Appended Dansyl‐Modified β ‐Cyclodextrin
Author(s) -
Ikunaga Taizo,
Ikeda Hiroshi,
Ueno Akihiko
Publication year - 1999
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/(sici)1521-3765(19990903)5:9<2698::aid-chem2698>3.0.co;2-g
Subject(s) - avidin , cyclodextrin , fluorescence , biotin , chemistry , biotinylation , ternary complex , combinatorial chemistry , biophysics , organic chemistry , biochemistry , enzyme , physics , quantum mechanics , biology
Fluorescence intensities enhanced by more than three times by the presence of avidin and considerable increases in the guest binding as a result of the promoted stability of the formed complexes. These are the main results from the sudies on biotin‐appended dansyl‐modified β ‐cyclodextrins, which were prepared to investigate the effects of avidin binding on the guest inclusion phenomena and the fluorescence properties of the cyclodextrin derivatives, such as 1 , and demonstrate that avidin acts as an effective hydrophobic cap in the ternary complex of [avidin]–[CD host]–[guest].