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D ‐Altritol Nucleic Acids (ANA): Hybridisation Properties, Stability, and Initial Structural Analysis
Author(s) -
Allart Brigitte,
Khan Kamran,
Rosemeyer Helmut,
Schepers Guy,
Hendrix Chris,
Rothenbacher Klaus,
Seela Frank,
Van Aerschot Arthur,
Herdewijn P.
Publication year - 1999
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/(sici)1521-3765(19990802)5:8<2424::aid-chem2424>3.0.co;2-w
Subject(s) - oligonucleotide , antiparallel (mathematics) , random hexamer , rna , dna , nucleic acid , duplex (building) , nucleobase , sequence (biology) , stereochemistry , complementary sequences , chemistry , crystallography , biology , biochemistry , gene , physics , combinatorics , mathematics , quantum mechanics , magnetic field
Oligonucleotides composed of a phosphorylated D ‐altritol backbone (see diagram) with nucleobases in the 2′‐position, hybridise strongly and sequence selectively with RNA in an antiparallel way (dsANA>ANA:RNA>ANA:DNA). Complexes between ANA and RNA or DNA are more stable than between HNA and natural oligonucleotides. A hexamer dsANA hybrid gives a Δ T m per modification of +10.2 °C relative to an identical dsDNA sequence. CD spectral analysis indicates that ANA complexes are very similar to the A‐form dsRNA duplex.