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Time‐Resolved Dynamics of the Vesicle Membrane During Individual Exocytotic Secretion Events, as Extracted from Amperometric Monitoring of Adrenaline Exocytosis from Chromaffin Cells
Author(s) -
Amatore Christian,
Bouret Yann,
Midrier Laurent
Publication year - 1999
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/(sici)1521-3765(19990702)5:7<2151::aid-chem2151>3.0.co;2-r
Subject(s) - exocytosis , amperometry , secretion , vesicle , secretory vesicle , chemistry , biophysics , microbiology and biotechnology , membrane , biology , biochemistry , electrode , electrochemistry
A theoretical description of the opening of adrenal cell vesicles during exocytosis (schematically depicted here) is proposed. Single secretion events measured amperometrically can thus be treated with a much better resolution than achievable by patch‐clamp techniques. It is concluded that the exocytosis is driven by swelling of the polyelectrolyte gel matrix of the secretory vesicle.