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Cyclic Oligoribonucleotides (RNA) by Solid‐Phase Synthesis
Author(s) -
Micura Ronald
Publication year - 1999
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/(sici)1521-3765(19990702)5:7<2077::aid-chem2077>3.0.co;2-u
Subject(s) - solid phase synthesis , combinatorial chemistry , rna , chemistry , cleavage (geology) , nucleic acid , biochemistry , biology , peptide , paleontology , fracture (geology) , gene
No restrictions on the sequence variety is one of the key features of the presented solid‐phase synthesis for small‐ to medium‐sized cyclic oligoribonucleotides (see diagram). The approach allows fully automated assembly with 2′‐ O ‐triisopropylsilyloxymethyl (TOM) RNA phosphoramidites. Ring closure is achieved by standard phosphotriester RNA chemistry. Selective cleavage from the solid support provides the cyclic oligoribonucleotide in high purity after deprotection.