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Syntheses of Nucleosides Designed for Combinatorial DNA Sequencing
Author(s) -
Welch Mike B.,
Martinez Carlos I.,
Zhang Alex J.,
Jin Song,
Gibbs Richard,
Burgess Kevin
Publication year - 1999
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/(sici)1521-3765(19990301)5:3<951::aid-chem951>3.0.co;2-g
Subject(s) - computational biology , dna sequencing , dna , computer science , combinatorial chemistry , biology , chemistry , genetics
Fluorescent and photolabile nucleotide analogues 1 and 2 were prepared to test an unconventional approach to DNA sequencing that, ideally, would not involve gel electrophoresis. These particular compounds were not incorporated, but modeling studies indicate how further refinements to their structures could facilitate the proposed sequencing scheme. B = adenine, thymine; TP = triphosphate,