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Synthesis, Crystal Structure, and Enzymatic Evaluation of a DNA‐Photolesion Isostere
Author(s) -
Butenandt Jens,
Eker André P. M.,
Carell Thomas
Publication year - 1998
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/(sici)1521-3765(19980416)4:4<642::aid-chem642>3.0.co;2-k
Subject(s) - pyrimidine dimer , oligonucleotide , dna , enzyme , dna repair , photolyase , dimer , isostere , chemistry , in vivo , biochemistry , dna damage , stereochemistry , biology , genetics , organic chemistry
UV irradiation of cells causes the formation of genotoxic pyrimidine dimer DNA lesions, which are repaired in vivo by various repair enzymes. The synthesis, X‐ray crystal structure, and incorporation into oligonucleotides of the lesion analogue 1 are described. Analysis of the reparability of oligonucleotides containing 1 with various DNA photolyase repair enzymes shows the efficient repair of such oligonucleotides, which proves that 1 is a useful tool for the investigation of DNA repair processes on a molecular level.