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Enzymatic Manipulation of the Fragments Obtained by Cerium( IV )‐Induced DNA Scission: Characterization of Hydrolytic Termini
Author(s) -
Sumaoka Jun,
Azuma Yasushi,
Komiyama Makoto
Publication year - 1998
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/(sici)1521-3765(19980210)4:2<205::aid-chem205>3.0.co;2-d
Subject(s) - terminal deoxynucleotidyl transferase , chemistry , dna , oligomer , bond cleavage , enzyme , polynucleotide , hydrolysis , enzymatic hydrolysis , transferase , biochemistry , cleavage (geology) , stereochemistry , polymer chemistry , biology , tunel assay , catalysis , apoptosis , paleontology , fracture (geology)
Direct evidence for the hydrolysis of DNA oligomers by Ce IV has been obtained. Three natural enzymes (bacterial alkaline phosphatase, T 4 polynucleotide kinase, and terminal deoxynucleotidyl transferase) quantitatively transformed the fragments obtained by the scission of the DNA oligomer with [Ce(NH 4 ) 2 (NO 3 ) 6 ] into the expected forms (see scheme below).