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Polyamine Analogues of 3 β ‐[ N‐ ( N′ , N′ ‐Dimethylaminoethane)carbamoyl]cholesterol (DC‐Chol) as Agents for Gene Delivery
Author(s) -
Cooper Robert G.,
Etheridge Christopher J.,
Stewart Luisa,
Marshall John,
Rudginsky Samantha,
Cheng Seng H.,
Miller Andrew D.
Publication year - 1998
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/(sici)1521-3765(199801)4:1<137::aid-chem137>3.0.co;2-2
Subject(s) - polyamine , liposome , cationic polymerization , cationic liposome , in vivo , gene delivery , in vitro , genetic enhancement , chemistry , biochemistry , biophysics , combinatorial chemistry , biology , gene , polymer chemistry , genetics
Gene delivery with cationic liposomes formed from the diamine‐lipid DC‐Chol and the neutral lipid DOPE has proven successful. New polyamine analogues of DC‐Chol have been synthesised, formulated into cationic liposomes with DOPE and biologically tested in vitro and in vivo in mouse lung. Cationic liposomes containing the novel DC‐Chol analogue CTAP (shown below) work 100 times more effectively in vivo than DC‐Chol containing liposomes (500 times better than DNA alone) and therefore should be suitable for use in human gene therapy approaches towards lung disorders and other clinical conditions.