Premium
Liver repopulation with hepatocyte transplantation: new avenues for gene and cell therapy
Author(s) -
Gupta Sanjeev,
Malhi Harmeet,
Gagandeep S.,
Novikoff Phyllis
Publication year - 1999
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/(sici)1521-2254(199911/12)1:6<386::aid-jgm73>3.0.co;2-z
Subject(s) - genetic enhancement , hepatocyte , repopulation , biology , transplantation , cancer research , gene , immunology , liver transplantation , stem cell , microbiology and biotechnology , medicine , genetics , in vitro , haematopoiesis
Liver‐directed gene therapy is appropriate for many conditions. Recent work established that liver repopulation with transplanted cells can be effective in treating genetic disorders. Although hepatocytes express therapeutic genes with considerable efficiency, correction of genetic disorders is constrained by limitations in permanent gene transfer into hepatocytes and repopulation of the liver with transplanted cells. Adenoviral vectors are highly efficient for hepatic gene transfer but the onset of deleterious host immune responses against adenoviral vectors, along with clearance of transduced hepatocytes have caused problems. Nonetheless, recent work concerning engraftment and proliferation of transplanted hepatocytes in the liver has provided significant new information, which should refocus interest in hepatocyte‐based therapies. Moreover, hepatocyte transplantation systems offer creative tools for defining critical mechanisms in gene regulation and survival of transduced cells. Copyright © 1999 John Wiley & Sons, Ltd.