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Observations on the effects of low frequency electromagnetic fields on cellular transcription in Drosophila larvae reared in field‐free conditions
Author(s) -
Tipping David R.,
Chapman Karen E.,
Birley Andrew J.,
Anderson Moray
Publication year - 1999
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/(sici)1521-186x(1999)20:2<129::aid-bem8>3.0.co;2-q
Subject(s) - electromagnetic field , biology , larva , transcription (linguistics) , anatomy , physics , botany , quantum mechanics , linguistics , philosophy
Drosophila larvae reared inside a μ‐metal box with an internal field strength 0.004 μT, were treated with a magnetic field of 50 Hz, 8 μT, for 20 min. Control experienced 0.004 μT. Cellular transcript levels were assessed using slot blots and quantified using a Phosphorimager. Blots were hybridised using probes against HSP 70a, Histone 1.9 , and Copia . The low frequency EMFs very significantly decreased transcript levels, indicating that experimental responses may be influenced by previous exposure or lack of previous exposure. Bioelectromagnetics 20:129–131, 1999. © 1999 Wiley‐Liss, Inc.