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Effects of extremely low frequency electromagnetic field (EMF) on collagen type I mRNA expression and extracellular matrix synthesis of human osteoblastic cells
Author(s) -
Heermeier K.,
Spanner M.,
Träger J.,
Gradinger R.,
Strauss P. G.,
Kraus W.,
Schmidt J.
Publication year - 1998
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/(sici)1521-186x(1998)19:4<222::aid-bem4>3.0.co;2-3
Subject(s) - extracellular matrix , type i collagen , osteoblast , alkaline phosphatase , osteocalcin , endocrinology , transforming growth factor beta , growth factor , medicine , extracellular , cell culture , chemistry , transforming growth factor , biology , microbiology and biotechnology , biochemistry , in vitro , enzyme , receptor , genetics
Abstract Human osteoblastic cells were grown in a three‐dimensional (3‐D) cell culture model and used to test the effects of a 20 Hz sinusoidal electromagnetic field (EMF; 6 mT and 113 mV/cm max) on collagen type I mRNA expression and extracellular matrix formation in comparison with the effects of growth factors. The cells were isolated from trabecular bone of a healthy individual (HO‐197) and from a patient presenting with myositis ossificans (MO‐192) and grown in a collagenous sponge‐like substrate. Maximal enhancement of collagen type I expression after EMF treatment was 3.7‐fold in HO‐197 cells and 5.4‐fold in MO‐192 cells. Similar enhancement was found after transforming growth factor‐β (TGF‐β) and insulin‐like growth factor‐I (IGF‐I) treatment. Combined treatment of the cells with EMF and the two growth factors TGF‐β and IGF‐I did not act synergistically. MO‐192 cells produced an osteoblast‐characteristic extracellular matrix containing collagen type I, alkaline phosphatase, and osteocalcin, together with collagen type III, TP‐1, and TP‐3, two epitopes of an osteoblastic differentiation marker. The data suggest that the effects of EMFs on osteoblastic differentiation are comparable to those of TGF‐β and IGF‐I. We conclude that EMF effects in the treatment of skeletal disorders and in orthopedic adjuvant therapy are mediated via enhancement of collagen type I mRNA expression, which may lead to extensive extracellular matrix synthesis. Bioelectromagnetics 19:222–231, 1998. © 1998 Wiley‐Liss, Inc.