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Cell membrane lipid molecular dynamics in a solenoid versus a magnetically shielded room
Author(s) -
Volpe P.,
Parasassi T.,
Esposito C.,
Ravagnan G.,
Giusti A. M.,
Pasquarelli A.,
Eremenko T.
Publication year - 1998
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/(sici)1521-186x(1998)19:2<107::aid-bem8>3.0.co;2-5
Subject(s) - shielded cable , solenoid , dynamics (music) , biophysics , membrane , nanotechnology , chemistry , biology , physics , materials science , biochemistry , engineering , electrical engineering , acoustics , quantum mechanics
The generalized polarization function of the fluorescent probe 2‐dimethylamino‐6‐lauroylnaphthalene has been used to evaluate the lipid dynamics in Friend erythroleukemia cell membrane. The values of this function varied during the culture growth cycle, showing decreased lipid dynamics 24–48 h from the cell seeding. When the cycle occurred in a solenoid producing a magnetic field of 70 μT at 50 Hz in addition to the 45 μT DC of the earth (short‐term 4‐day exposure), the membrane lipid dynamics during this same time‐period decreased by about 10% ( P < .04). After long‐term (184 days) or extremely long‐term (395 days) exposure of the cells to the magnetic field, little additional variation in the membrane lipid dynamics was observed, suggesting an adaptation phenomenon. A variation of membrane lipid dynamics was also observed due to in vitro cell differentiation ( P < .02). Nevertheless, the exposure of both undifferentiating and differentiating cells to a highly attenuated magnetic field in a magnetically shielded room (20 nT DC plus 2.5 pT AC) did not induce any modification of membrane lipid dynamics. Bioelectromagnetics 19:107–111, 1998. © 1998 Wiley‐Liss, Inc.