Premium
Action of extremely low frequency electric fields on the cytosolic calcium concentration of differentiated HL‐60 cells: Nonactivated cells
Author(s) -
Sontag W.
Publication year - 1998
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/(sici)1521-186x(1998)19:1<32::aid-bem3>3.0.co;2-5
Subject(s) - cuvette , fluorescence , calcium , electric field , cytosol , analytical chemistry (journal) , chemistry , biophysics , spectrometer , electrode , materials science , nuclear magnetic resonance , chromatography , optics , biochemistry , biology , physics , organic chemistry , quantum mechanics , enzyme
The effect of sinusoidal electric fields on the cytosolic free [Ca 2+ ] i concentration in differentiated HL‐60 cells was measured. The calcium concentration was measured in a fluorescence spectrometer using the fluorescence sample fluo‐3. In the fluorescence spectrometer two samples can be measured simultaneously, one as the sham‐exposed control and the other as the field‐exposed sample. The effects of an external field, applied using two capacitor plates outside the cuvettes, and a field applied directly to the medium, using two platinum electrodes inside the cuvettes, were measured at selected frequencies between 0 and 100 Hz and field strengths from 1 to 2000 V pp /m (external field) and from 0.1 to 1000 V pp /m (in medium). No significant effects of the fields on the cytosolic free [Ca 2+ ] i concentration in HL‐60 cells have been observed at the measured frequencies and field strengths. Bioelectromagnetics 19:32–40, 1998. © 1998 Wiley‐Liss, Inc.