Premium
Stress proteins are not induced in mammalian cells exposed to radiofrequency or microwave radiation
Author(s) -
Cleary Stephen F.,
Cao Guanghui,
Liu LiMing,
Egle Patsy M.,
Shelton Keith R.
Publication year - 1997
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/(sici)1521-186x(1997)18:7<499::aid-bem5>3.0.co;2-y
Subject(s) - hela , microwave , in vitro , microbiology and biotechnology , chemistry , biophysics , biology , biochemistry , physics , quantum mechanics
The induction of stress proteins in HeLa and CHO cells was investigated following a 2 h exposure to radiofrequency (RF) or microwave radiation. Cells were exposed or sham exposed in vitro under isothermal (37 ± 0.2 °C) conditions. HeLa cells were exposed to 27‐ or 2450 MHz continuous wave (CW) radiation at a specific absorption rate (SAR) of 25 W/kg. CHO cells were exposed to CW 27 MHz radiation at a SAR of 100 W/kg. Parallel positive control studies included 2 h exposure of HeLa or CHO cells to 40 °C or to 45 μM cadmium sulfate. Stress protein induction was assayed 24 h after treatment by electrophoresis of whole‐cell extracted protein labeled with [ 35 S]‐methionine. Both cell types exhibited well‐characterized responses to the positive control stresses. Under these exposure conditions, neither microwave nor RF radiation had a detectable effect on stress protein induction as determined by either comparison of RF‐exposed cells with sham‐exposed cells or comparison with heat‐stressed or Cd ++ positive control cells. Bioelectromagnetics 18:499–505, 1997. © 1997 Wiley‐Liss, Inc.