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Intracellular calcium signaling by jurkat T‐lymphocytes exposed to a 60 hz magnetic field
Author(s) -
Lyle Daniel B.,
Fuchs Thomas A.,
Casamento Jon P.,
Davis Christopher C.,
Swicord Mays L.
Publication year - 1997
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/(sici)1521-186x(1997)18:6<439::aid-bem6>3.0.co;2-3
Subject(s) - jurkat cells , calcium , calcium in biology , calcium signaling , intracellular , bioelectromagnetics , microbiology and biotechnology , chemistry , t cell , biophysics , biology , immune system , immunology , magnetic field , physics , organic chemistry , quantum mechanics
To explore possible biochemical mechanisms whereby electromagnetic fields of around 0.1 mT might affect immune cells or developing cancer cells, we studied intracellular calcium signaling in the model system Jurkat E6‐1 human T‐leukemia cells during and following exposure to a 60 Hz magnetic field. Cells were labeled with the intracellular calcium‐sensitive fluorescent dye Fluo‐3, stimulated with a monoclonal antibody against the cell surface structure CD3 (associated with ligand‐stimulated T‐cell activation), and analyzed on a FACScan flow‐cytometer for increases in intensity of emissions in the range of 515–545 nm. Cells were exposed during or before calcium signal‐stimulation to 0.15 mT rms 60 Hz magnetic field. The total DC magnetic field of 78.2 μT was aligned 17.5° off the vertical axis. Experiments used both cells cultured at optimal conditions at 37 °C and cells grown under suboptimal conditions of 24 °C, lowered external calcium, or lowered anti‐CD3 concentration. These experiments demonstrate that intracellular signaling in Jurkat E6‐1 was not affected by a 60 Hz magnetic field when culture and calcium signal‐stimulation were optimal or suboptimal. These results do not exclude field‐induced calcium‐related effects further down the calcium signaling pathway, such as on calmodulin or other calcium‐sensitive enzymes. Bioelectromagnetics 18:439–445, 1997. © 1997 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.