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Smooth muscle electromyography of the urinary bladder
Author(s) -
Scheepe J.R.,
Wipfler G.,
Schumacher S.,
Bross S.,
Zendler S.,
Jünemann K.P.,
Alken P.
Publication year - 1998
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/(sici)1520-6777(1998)17:1<71::aid-nau9>3.0.co;2-a
Subject(s) - medicine , electromyography , urinary bladder , urology , urinary system , physical medicine and rehabilitation , anatomy
To elucidate smooth muscle activity of the urinary bladder, we utilized an optimized animal model and a specially developed, computer‐aided data acquisition and analysis system for bioelectrical signals. Twenty‐five Wistar rats were pharmacologically paralyzed and artificially respirated. The urinary bladder was exposed by a suprapubic midabdominal incision, and both ureters were ligated to prevent physiological filling of the bladder. The bladder was initially emptied by slight manual pressure and was then filled via a transurethral catheter in 0.1‐ml steps to a maximum of 0.45 ml with physiological saline. A custom‐made, gold‐plated needle electrode was tangentially guided by a micromanipulator to the smooth muscle of the bladder dome, and the recordings commenced. Furthermore, smooth muscle EMG recordings of the bladder were performed after pharmaco‐stimulation of the detrusor with carbachol. Initial results demonstrate that, with the animal model presented here, it is possible to record reproducible and almost artifact‐free smooth muscle activity from the urinary bladder. All experiments displayed a stochastic distribution of similar electrical events, increasing in appearance and amplitude with increased bladder volume and after pharmaco‐stimulation with carbachol. Two‐dimensional power spectrum analysis revealed a main signal frequency below 1 Hz. Neurourol. Urodynam. 17:71–83, 1998. © 1998 Wiley‐Liss, Inc.