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Effective range of electrical stimulation in brain slice preparations
Author(s) -
Takimori Tohru,
Ogawa Tetsuro,
Nishida Makoto
Publication year - 1999
Publication title -
electrical engineering in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.136
H-Index - 28
eISSN - 1520-6416
pISSN - 0424-7760
DOI - 10.1002/(sici)1520-6416(19990415)127:1<56::aid-eej7>3.0.co;2-6
Subject(s) - excitatory postsynaptic potential , stimulus (psychology) , stimulation , slice preparation , inhibitory postsynaptic potential , neuron , neuroscience , materials science , electrophysiology , biology , psychology , psychotherapist
In order to examine the confines of electrical stimulation in layer 2/3 of the visual cortex in brain slice preparations, we estimated the effective range of stimulation based on the excitatory postsynaptic potential (EPSP) evoked in layer V neurons that receive input from layer 2/3. For this purpose, we recorded and compared ESPS amplitudes evoked by stimulations directly over the site of the recording electrode and at a lateral site in layer 2/3. Since the EPSP increased linearly with stimulus intensity before saturation, it was considered that the EPSP correlates with the number of projecting neurons in an area directly excited by stimulation. Then we formed a region model by which we can obtain the ratios between the neuron numbers in areas excited by different site stimulations against the stimulus effective ranges. In the stimulus intensity for the action potential threshold of layer 5 neurons, we evaluated the effective range for the relative values of EPSP produced by stimulations 250‐μm lateral sites and directly over the site. In the model, the ratio increased monotonically with the effective range and in the case of 250 μm for the effective range, the ratio between these EPSP was less than the value in the model. These results led to the conclusion that the effective range of the intensity for layer 5 neurons to generate an output is confined within 250 μm of a point directly over the site, that is, within layer 2/3. © 1999 Scripta Technica, Electr Eng Jpn, 127(1): 56–63, 1999