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Circular dichroism as a detection method in the screening of enantioselective catalysts
Author(s) -
Reetz Manfred T.,
Kühling Klaus M.,
Hinrichs Heike,
Deege Alfred
Publication year - 2000
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/(sici)1520-636x(2000)12:5/6<479::aid-chir32>3.0.co;2-#
Subject(s) - enantioselective synthesis , chemistry , circular dichroism , catalysis , combinatorial chemistry , stereochemistry , organic chemistry
The combination of liquid chromatography (HPLC), UV/Vis‐spectroscopy and circular dichroism (CD) can be used to construct a high‐throughput screening system to determine the enantioselectivity of enzyme‐ or metal‐catalyzed reduction of acetophenone with formation of ( S )‐ and ( R )‐1‐phenylethanol. Prerequisite for the viability of this system is the experimental finding that the anisotropy factor g is linearly related to the enantiomeric excess (ee) and that it is independent of concentration, thereby excluding possible aggregation effects. Chirality 12:479–482, 2000. © 2000 Wiley‐Liss, Inc.