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Enantioseparations with the macrocyclic antibiotic ristocetin a using a countercurrent process in CE
Author(s) -
Oswald Tanya M.,
Ward Timothy J.
Publication year - 1999
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/(sici)1520-636x(1999)11:8<663::aid-chir9>3.0.co;2-v
Subject(s) - chemistry , selectivity , countercurrent exchange , chromatography , countercurrent distribution , combinatorial chemistry , organic chemistry , catalysis , physics , thermodynamics
The chiral selectivity of ristocetin A was examined in a countercurrent process in CE using a coated column to suppress electroosmotic flow. Excellent enantioseparations of several nonsteroidal antiinflammatories, dansylamino acids, dinitrophenyl‐derivatized amino acids, and other optically active compounds were achieved. The chiral selectivity of ristocetin A also was examined as a function of antibiotic concentration and pH. Enantioresolution was found to significantly improve with a slight increase in migration time upon increasing chiral selector concentration. Enantioselectivities were found to be greatly influenced by pH of the running buffer. Chirality 11:663–668, 1999. © 1999 Wiley‐Liss, Inc.