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Enantioselective determination of isradipine in human serum using chiral stationary phase liquid chromatography and gas chromatography with nitrogen selective detection
Author(s) -
Rask Heidi S.,
Angelo Helle R.,
Christensen Hanne R.
Publication year - 1998
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/(sici)1520-636x(1998)10:9<808::aid-chir6>3.0.co;2-h
Subject(s) - isradipine , chemistry , chromatography , enantiomer , dihydropyridine , gas chromatography , enantioselective synthesis , chirality (physics) , calcium , stereochemistry , organic chemistry , catalysis , nambu–jona lasinio model , chiral symmetry breaking , physics , quark , quantum mechanics
rac ‐Isradipine is a dihydropyridine type calcium antagonist. Its calcium entry blocking effect is due primarily to the (+)‐(S)‐enantiomer. This study describes a sensitive enantioselective method for the determination of isradipine in human serum. Following alkaline extraction into hexane, the enantiomers of isradipine are separated quantitatively by high‐performance liquid chromatography on a Chiralcel OJ column at 39°C. The collected fractions were evaporated and assayed using capillary gas chromatography on a HP 50+ column with nitrogen selective detection. Using 2.0 ml of serum, 0.7 nmol/1 (0.26 ng/ml) of each enantiomer could be determined with acceptable precision. The method has successfully been used to measure (+)‐(S)‐ and (−)‐(R)‐isradipine concentrations in samples from volunteers after intravenous and oral administration of isradipine. Chirality 10:808–812, 1998. © 1998 Wiley‐Liss, Inc.