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Determination of the enantiomers of albuterol in human and canine plasma by enantioselective high‐performance liquid chromatography on a teicoplanin‐based chiral stationary phase
Author(s) -
Fried Karen M.,
Koch Patrick,
Wainer Irving W.
Publication year - 1998
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/(sici)1520-636x(1998)10:5<484::aid-chir11>3.0.co;2-w
Subject(s) - chemistry , enantiomer , chromatography , diethylamine , enantioselective synthesis , high performance liquid chromatography , racemic mixture , teicoplanin , chirality (physics) , analytical chemistry (journal) , organic chemistry , chiral symmetry breaking , physics , quantum mechanics , vancomycin , quark , biology , bacteria , nambu–jona lasinio model , genetics , staphylococcus aureus , catalysis
A sensitive enantioselective high‐performance chromatographic (HPLC) method was developed and validated to determine low levels of (−)‐R and (+)‐S‐albuterol in plasma. Baseline resolution was achieved by using a teicoplanin‐based chiral stationary phase with a polar organic mobile phase consisting of methanol/acetonitrile/glacial acetic acid/diethylamine, 40:60:0.3:0.2, (v/v/v/v) and a flow‐rate of 1.0 ml/min. Enantioselectivity (α) equaled 1.18 and resolution (R s ) equaled 1.8. By using fluorescence detection maximized at 230 and 310 nm for excitation and emission, respectively, concentrations of each enantiomer could be measured down to 125 pg/ml from a 1‐ml plasma sample. Initially, the method was applied to plasma samples from a small single‐dose inhalation study of racemic albuterol in a human volunteer and, later, to in vivo samples from a canine inhalation study of the single enantiomer, (−)‐R‐albuterol. Results from the canine study showed that no chiral inversion of (−)‐R‐albuterol occurs in the dog. Chirality 10:484–491, 1998. © 1998 Wiley‐Liss, Inc.