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Highly enantioselective HPLC separations using the covalently bonded macrocyclic antibiotic, ristocetin A, chiral stationary phase
Author(s) -
EkborgOtt K. Helen,
Liu Youbang,
Armstrong Daniel W.
Publication year - 1998
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/(sici)1520-636x(1998)10:5<434::aid-chir10>3.0.co;2-0
Subject(s) - chemistry , teicoplanin , glycopeptide , chromatography , enantioselective synthesis , chiral column chromatography , phase (matter) , glycopeptide antibiotic , selectivity , high performance liquid chromatography , covalent bond , chiral stationary phase , silica gel , ristocetin , organic chemistry , combinatorial chemistry , vancomycin , antibiotics , catalysis , biochemistry , platelet , platelet aggregation , biology , bacteria , immunology , genetics , staphylococcus aureus
The macrocyclic glycopeptide, ristocetin A, was covalently bonded to a silica gel support and evaluated as a liquid chromatographic (LC) chiral stationary phase (CSP). Over 230 racemates were resolved in either the reversed‐phase mode, the normal‐phase mode, or the polar‐organic mode. The retention behavior and selectivity of this CSP were examined in each mode. Optimization of separations on this column is discussed. The ristocetin A CSP appeared to be complimentary to other glycopeptide CSPs (i.e., vancomycin and teicoplanin). Column stability was excellent. The CSP was not irreversibly altered when going from one mobile phase mode to another. Chirality 10:434–483, 1998. © 1998 Wiley‐Liss, Inc.