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Manipulation of chiral resolution for isoxazoline‐based IIb/IIIa receptor antagonists using various mobile phase additives in subcritical fluid chromatography
Author(s) -
Blackwell John A.
Publication year - 1998
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/(sici)1520-636x(1998)10:4<338::aid-chir8>3.0.co;2-3
Subject(s) - chemistry , analyte , enantiomer , resolution (logic) , chirality (physics) , chiral derivatizing agent , phase (matter) , chiral resolution , chiral column chromatography , chromatography , methanol , combinatorial chemistry , organic chemistry , chiral symmetry , artificial intelligence , computer science , physics , quantum mechanics , nambu–jona lasinio model , quark
Subcritical fluid chromatography (SubFC) using a carbon dioxide‐methanol mobile phase is used for the chiral resolution of IIb/IIIa receptor antagonist enantiomers. The chiral resolution of three analogs, each containing two chiral centers, is optimized using various mobile phase additives. The effects that acidic, basic, and neutral additives have on retention, efficiency, and resolution are examined. The additive that gives the best resolution was found to be dependent upon the functionality and charge of the chiral analyte. For charged analytes, additives that act as competing ions of the same charge as the chiral analyte dramatically improve efficiency and resolution. Resolution of neutral chiral analyte enantiomers is also greatly affected by the choice of mobile phase additive. Chirality 10:338–342, 1998. © 1998 Wiley‐Liss, Inc.