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Enantioselective analysis of albendazole sulfoxide in plasma using the chiral stationary phase
Author(s) -
Paias Fernanda Orsi,
Lanchote Vera Lúcia,
Takayanagui Osvaldo Massaiti,
Bonato Pierina Sueli
Publication year - 1997
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/(sici)1520-636x(1997)9:8<722::aid-chir2>3.0.co;2-7
Subject(s) - chemistry , enantiomer , chromatography , enantioselective synthesis , sulfoxide , albendazole , chirality (physics) , chloroform , pharmacokinetics , stereochemistry , organic chemistry , pharmacology , catalysis , chiral symmetry breaking , medicine , nambu–jona lasinio model , surgery , physics , quantum mechanics , quark
We present a method for the enantioselective analysis of albendazole sulfoxide (ABZSO) in plasma for application in clinical pharmacokinetic studies. ABZSO enantiomers were separated on a 5‐μm Chiralcel OB‐H® column (4.6 × 150 mm) using hexane:ethanol (93:7, v/v) as the mobile phase and fluorescence detection. ABZSO was extracted with chloroform:isopropanol (8:2, v/v) from 500‐μl aliquots of acidified plasma, with full drug recovery. The proposed method presented quantitation limits of 20 ng/ml for (−)ABZSO and 50 ng/ml for (+)ABZSO and was linear up to a concentration of 5,000 ng/ml of each enantiomer. Chirality 9:722–726, 1997. © 1997 Wiley‐Liss, Inc.