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Enantioselective determination of FCE 28833, a new potential antiischemic agent, in gerbil plasma using column switching HPLC with UV detection
Author(s) -
Breda M.,
Sarati S.,
Basileo G.,
Dostert P.
Publication year - 1997
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/(sici)1520-636x(1997)9:2<133::aid-chir10>3.0.co;2-o
Subject(s) - chemistry , chromatography , enantiomer , elution , high performance liquid chromatography , detection limit , cartridge , solid phase extraction , extraction (chemistry) , analytical chemistry (journal) , stereochemistry , mechanical engineering , engineering
A sensitive and selective high performance liquid chromatographic method using an automated column switching technique for the determination of FCE 28833 enantiomers in gerbil plasma was developed. After solid‐liquid extraction using a Supelcosil C 18 cartridge FCE 28833 was eluted on a clean‐up column (Spherisorb CN) and the enantiomers were separated using an analytical chiral column (Crownpack CR(+)). The mobile phase (15% methanol in HClO 4 1 mM) was directed through the columns at a flow rate of 1 ml/min and the fraction eluted between 13 and 40 min was transferred from the clean‐up column into the analytical column. FCE 28833 enantiomers were monitored at 257 nm. The limit of quantitation of the method was 20 ng/ml plasma for both enantiomers and proved to be linear, precise, and accurate for the assay of both enantiomers in the 20–6,000 ng/ml concentration range. No interference from the blank gerbil plasma sample was observed. The suitability of the method was assessed using plasma samples obtained from male gerbils treated with a single oral dose (400 mg/kg) of FCE 28833. Chirality 9:133–138, 1997. © 1997 Wiley‐Liss, Inc.