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Optical rotation detection for reversed phase HPLC: Investigation of solvent effects
Author(s) -
Sims Jonathan L.,
Carreira Judith A.,
Bragg Ryan A.
Publication year - 1997
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/(sici)1520-636x(1997)9:2<122::aid-chir8>3.0.co;2-j
Subject(s) - chemistry , optical rotation , high performance liquid chromatography , rotation (mathematics) , phase (matter) , chromatography , solvent , solvent effects , reversed phase chromatography , chiral stationary phase , organic chemistry , artificial intelligence , computer science
Investigations into the parameters affecting the sensitivity of polarimetric detectors for the determination of enantiomeric excess by achiral HPLC are presented. The results obtained showed that the specific optical rotation of an analyte was highly sensitive to the methanol content of the mobile phase. The purpose of this short communication is to demonstrate that “off‐line” optical rotation measurements are a necessary part of the method development process for successful use of polarimetric detectors. Chirality 9:122–125, 1997, © 1997 Wiley‐Liss, Inc.