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Sulfated cyclodextrins for the chiral separations of catecholamines and related compounds in the reversed electrophoretic polarity mode
Author(s) -
Gahm KyungHyun,
Stalcup Apryll M.
Publication year - 1996
Publication title -
chirality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 77
eISSN - 1520-636X
pISSN - 0899-0042
DOI - 10.1002/(sici)1520-636x(1996)8:4<316::aid-chir5>3.0.co;2-d
Subject(s) - chemistry , capillary electrophoresis , monobasic acid , phenylalanine , polarity (international relations) , sulfation , cyclodextrin , electrophoresis , phosphoric acid , chromatography , organic chemistry , biochemistry , amino acid , cell
The utility of negatively charged sulfated cyclodextrins (SCD) as chiral additives (CA) in capillary electrophoresis (CE) was studied in the chiral resolution of several compounds of pharmaceutical interest, including catecholamines such as norepinephrine, epinephrine, DOPA and their precursors, phenylalanine, and tyrosine. Experiments were conducted using 10 mM sodium phosphate monobasic solution and 2% SCD adjusted to pH 3.2 with phosphoric acid. Chiral recognition mechanisms were explored using structurally related analytes including basic, acidic, and neutral compounds as well as 3,5‐dinitrobenzoyl phenylglycine, phenylalanine, and homophenylalanine. The advantage of the reversed electrophoretic polarity mode for the enantioresolution of these compounds is also discussed. © 1996 Wiley‐Liss, Inc.