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Fatty acid incorporation by Rhodnius prolixus midgut
Author(s) -
Atella Geórgia C.,
Arruda Maria Augusta B.C.F.,
Masuda Hatisaburo,
Gondim Katia C.
Publication year - 2000
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(200003)43:3<99::aid-arch1>3.0.co;2-3
Subject(s) - rhodnius prolixus , midgut , oleic acid , blood meal , biology , hemolymph , biochemistry , context (archaeology) , diacylglycerol kinase , fatty acid , rhodnius , meal , insect , food science , botany , enzyme , paleontology , protein kinase c , larva
[ 14 C]Oleic acid injected into the hemocoel of Rhodnius prolixus females was shown to rapidly associate with lipophorin particles. Half of the lipophorin‐associated [ 14 C]oleic acid was transferred in about 5 min to different organs, but the midgut was the main organ to take it up on day 10 after a blood meal. The rate of [ 14 C]oleic acid incorporation by the midgut was high up to 15 min after injection and then declined. The [ 14 C]oleic acid incorporated by the midgut was found in phospholipids (58.6%) and neutral lipids (37.4%). The midgut capacity to incorporate [ 14 C]oleic acid varied on different days after a meal: it increased up to day 10 and then decreased. The fate of the [ 14 C]lipids synthesized by the midgut was followed and it was observed that 10 days after feeding diacylglycerol was the main lipid released to hemolymph and that most of phospholipids and triacylglycerols remained associated with the midgut. The metabolism of free fatty acids in Rhodnius prolixus females is discussed in the context of major biological events that follow a blood meal such as digestion and oogenesis. Arch. Insect Biochem. Physiol. 43:99–107, 2000. © 2000 Wiley‐Liss, Inc.