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Phytoecdysteroids in seeds and plants of Rhagodia baccata (Labill.) Moq. (Chenopodiaceae)
Author(s) -
Dinan Laurence,
Sarker Satyajit D.,
Bourne Pauline,
Whiting Pensri,
Šik Vladimir,
Rees Huw H.
Publication year - 1999
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1999)41:1<18::aid-arch4>3.0.co;2-t
Subject(s) - ecdysteroid , 20 hydroxyecdysone , biology , botany , bioassay , sesquiterpene , taxus , larva , ecology
Seeds of Rhagodia baccata afforded, in addition to 20‐hydroxyecdysone and polypodine B, a novel phytoecdysteroid, (20 R )‐22‐deoxy‐20,21‐dihydroxyecdysone, the structure of which was elucidated unequivocally by UV, LSIMS and NMR techniques. This compound possessed agonistic activity in the Drosophila melanogaster B II cell bioassay, with an ED 50 value of 2.0 × 10 –7 M (ED 50 value for 20‐hydroxyecdysone = 7.5 × 10 –9 M). The distribution of ecdysteroids in plants of R. baccata has been determined. Highest levels are associated with the youngest aerial tissues and with the roots. Ecdysteroid profiles are qualitatively very similar throughout the plant, with 20‐hydroxyecdysone and polypodine B predominating in all plant parts tested. Arch. Insect Biochem. Physiol. 41:18–23, 1999. © 1999 Wiley‐Liss, Inc.