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Distribution and accumulation of storage protein‐1 in ovary of Hyphantria cunea Drury
Author(s) -
Seo SookJae,
Kang YoungJin,
Cheon HyangMi,
Kim HakRyul
Publication year - 1998
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1998)37:2<115::aid-arch1>3.0.co;2-t
Subject(s) - hyphantria , biology , hemolymph , yolk , vitellogenesis , ovary , oocyte , methionine , storage protein , insect , methionine sulfoxide , medicine , biochemistry , endocrinology , andrology , amino acid , microbiology and biotechnology , botany , larva , ecology , embryo , gene
Storage protein‐1 (SP‐1) is a major storage protein found in the hemolymph and fat body of Hyphantria cunea . In this study, the uptake and accumulation of SP‐1 into the ovary of H. cunea was investigated using biochemical and immunocytochemical methods. SP‐1 in H. cunea has a high methionine content (4.6%) but is not female‐specific, like other high methionine storage proteins. In the 6‐day‐old pupal ovary, SP‐1 was detectable in trace amounts but accumulated to significant levels toward the end of the pupal stage. After adult emergence, SP‐1 rapidly decreased in the ovarian follicles and remained low in the egg. This suggest that SP‐1 is either extensively modified or degraded, causing a loss of its antigenic property in the ovary after adult emergence. During vitellogenesis, SP‐1 is present in the hemolymph and penetrates through the tunica propria to reach the perioocytic space. From there, SP‐1 is incorporated into yolk bodies. These results clearly show that SP‐1 is taken up by the developing oocyte. Its disappearance suggests that SP‐1 might be an amino acid reservoir for providing precursors for egg formation, in contrast to yolk proteins, which are utilized during postembryonic development. Arch. Insect Biochem. Physiol. 37:115–128, 1998. © 1998 Wiley‐Liss, Inc.