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Expression of neuroparsin cDNA in insect cells using baculovirus vectors
Author(s) -
Girardie J.,
Chaabihi H.,
Fournier B.,
Lagueux M.,
Girardie A.
Publication year - 1997
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1997)36:1<11::aid-arch2>3.0.co;2-z
Subject(s) - biology , complementary dna , baculoviridae , insect , vector (molecular biology) , microbiology and biotechnology , gene expression , computational biology , virology , gene , genetics , botany , recombinant dna , spodoptera
Abstract The cDNA encoding neuroparsin A, a polytropic neurohormone of the locust, Locusta migratoria , was inserted into the genome of Autographa californica nuclear polyhedrosis virus such that transcription was under control of the p10 promoter. A polypeptide having the same charge and the same apparent molecular weight as the authentic neuroparsin A and that was reactive against neuroparsin immune serum was produced in recombinant virus‐infected lepidopteran cell lines but not in control virus‐infected cells. The baculovirus‐expressed polypeptide was purified by two steps of liquid chromatography (anion exchange and reversed phase) which were previously used to purify the natural neuroparsin. The purified baculovirus‐expressed polypeptide enhanced fluid reabsorption of everted rectum preparations, as did the natural neuroparsin. Thus, this gene expression system produced a polypeptide identical to authentic neuroparsin. Arch. Insect Biochem. Physiol. 36:11–23, 1997.© 1997 Wiley‐Liss, Inc.