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Effects of ovariectomy and allatectomy on ecdysteroid synthesis and ecdysteroid titers during larval‐adult development of Gryllus bimaculatus de Geer (Ensifera: Gryllidae)
Author(s) -
Hoffmann Klaus H.,
Gerstenlauer Brigitte
Publication year - 1997
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1997)35:1/2<149::aid-arch13>3.0.co;2-b
Subject(s) - ecdysteroid , biology , medicine , integument , endocrinology , hemolymph , instar , vitellogenesis , gryllus bimaculatus , ovary , juvenile hormone , ovariectomized rat , larva , anatomy , zoology , hormone , botany , cricket , embryo , oocyte , microbiology and biotechnology
In last instar larvae of Gryllus bimaculatus , the prothoracic glands as well as the ovary and the abdominal integument synthesize and release ecdysteroids. Ovariectomy of day (d) 1 penultimate instar larvae extended the last larval stadium from 8 to 12 days (at 27°C). Ecdysteroid titers in haemolymph and abdominal integument of ovariectomized d 6/7 last instar larvae were similar to those of d 4 intact larvae and of d 6/7 sham‐operated animals. Net release of ecdysteroids (in vitro) by the abdominal integument from ovariectomized and sham‐operated d 6 last instar larvae was twice that for intact d 4 specimens. Ovariectomy of d 1 last instar larvae strongly reduced levels of ecdysteroids in haemolymph and integument as well as release of ecdysteroids by the abdominal integument in 4‐day‐old adults. Allatectomy of d 1 last instar larvae also markedly reduced ecdysteroid titers as well as rates of ecdysteroid release by the abdominal integument and by the ovary of young female crickets. The results suggest a prominent role of the ovary in ecdysteroid synthesis of adult females and indicate a juvenile hormone‐dependent control of ecdysteroid biosynthesis during vitellogenesis. Arch. Insect Biochem. Physiol. 35:149–158, 1997. © 1997 Wiley‐Liss, Inc.