Premium
Recombinant juvenile hormone esterase as a biochemical anti‐juvenile hormone agent: Effects on ovarian development in Acheta domesticus
Author(s) -
Bonning Bryony C.,
Loher Werner,
Hammock Bruce D.
Publication year - 1997
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1997)34:3<359::aid-arch9>3.0.co;2-p
Subject(s) - acheta , hemolymph , juvenile hormone , biology , recombinant dna , heliothis virescens , endocrinology , medicine , hormone , biochemistry , lepidoptera genitalia , botany , noctuidae , zoology , cricket , gene
By investigating the effects of recombinant juvenile hormone esterase (JHE) on the stimulation of ovarian development and egg laying in the house cricket Acheta domesticus L., we have tested the hypothesis that recombinant JHE (derived from the tobacco budworm Heliothis virescens ) can be used as a biochemical anti‐juvenile hormone (JH) agent. Recombinant JHE, produced by a genetically engineered baculovirus, was affinity‐purified and injected into females of A. domesticus . JHE was cleared rapidly from the hemolymph of the crickets. However, upon repeated injection, significant reductions were seen in the extent of development of the ovaries and in the numbers of eggs laid. The effects of JHE could be rescued by topical application of the JHE inhibitor, OTFP. Thus, we have demonstrated an anti‐JH effect on reproduction and that the recombinant JHE derived from a lepidopteran is active in an orthopteran insect. Arch. Insect Biochem. Physiol. 34:359–368, 1997. © 1997 Wiley‐Liss, Inc.