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Juvenile hormone synthesis by ring glands of the blowfly Lucilia cuprina
Author(s) -
East P.D.,
Sutherland T.D.,
Trowell S.C.,
Herlt A.J.,
Rickards R.W.
Publication year - 1997
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1997)34:2<239::aid-arch8>3.0.co;2-r
Subject(s) - juvenile hormone , lucilia cuprina , corpus allatum , biology , hemolymph , instar , endocrinology , medicine , methoprene , metamorphosis , larva , methionine , juvenile , hormone , pupa , salivary gland , biochemistry , amino acid , calliphoridae , botany , genetics
The major radiolabelled product released from ring gland and brain‐ring gland complexes of third instar larval and pre‐pupal stages of the sheep blowfly Lucilia cuprina upon incubation with L‐[ methyl ‐ 3 H]methionine corresponded to one diastereomer of juvenile hormone III bisepoxide (JHB 3 ). Endocrine glands incubated with the juvenile hormone precursor 2 E ,6 E ‐farnesoic acid released increased quantities of JHB 3 , together with significant amounts of juvenile hormone III but not the isomeric methyl 2 E ‐6,7‐epoxyfarnesoate. Synthesis of JHB 3 was developmentally and neurally regulated. Ring glands and brain‐ring gland complexes from third instar larvae released more JHB 3 than comparable preparations from pre‐pupae, while isolated corpus allatum segments of the gland were more active than intact brain‐gland complexes. These results reinforce the emerging status of JHB 3 as the characteristic juvenile hormone of dipteran insects. Arch. Insect Biochem. Physiol. 34:239–253, 1997. © 1997 Wiley‐Liss, Inc.