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Apparent inhibition of in vitro juvenile hormone biosynthesis by the corpus cardiacum of adult crickets ( Gryllus bimaculatus and Acheta domesticus ) is due to juvenile hormone esterase
Author(s) -
Woodring Joseph,
Hoffmann Klaus H.
Publication year - 1997
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1997)34:1<19::aid-arch2>3.0.co;2-r
Subject(s) - gryllus bimaculatus , corpus allatum , octopamine (neurotransmitter) , adipokinetic hormone , juvenile hormone , medicine , endocrinology , biology , acheta , orthoptera , in vitro , locust , biosynthesis , esterase , acrididae , hemolymph , cricket , hormone , biochemistry , enzyme , serotonin , neuropeptide , botany , receptor , ecology
When measuring the in vitro JH III‐biosynthesis by corpora allata (CA) from adult female crickets in the presence of corpora cardiaca (CC), the amount of JH III in the medium decreased in a dose dependent manner. The CC of a 4‐day‐old female Gryllus bimaculatus contain 42 pmol.pair CC −1 Grb‐AKH, 0.62 pmol.pair CC −1 octopamine, and a JH‐esterase activity of 9.8 pmol JH.h −1 .pair CC −1 . Comparable values for Acheta domesticus are 21 pmol.pair CC −1 Grb‐AKH, 0.53 pmol.pair CC −1 octopamine, and 6.5 pmolJH.h −1 .pair CC −1 of JH‐esterase activity. Even if the entire octopamine content of the CC were released into the medium, the concentration would be below the 10 −5 M threshold for octopamine inhibition of JH synthesis. An in vitro AKH inhibition of JH III synthesis was observed, but only at a relatively high concentration (10 −5 M). If the entire AKH content (10 −6 M) of the CC were released into the medium, the AKH concentration would approach JH synthesis inhibiting levels. However, the rate of release of AKH in vitro was very low, and, therefore, AKH from the CC could not affect JH synthesis. In contrast, a specific JH‐esterase, released by isolated CC into the medium, was sufficiently high in both cricket species to account for the observed decrease in JH III present. OTFP‐sulfone (10 −5 M) restored apparent JH synthesis of the CA to the control level. There was no reduction in the amount of JH released when CA were incubated with heat treated CC. The CA themselves contained almost no JH‐esterase activity. © 1997 Wiley‐Liss, Inc.