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Fenoxycarb and thyroid hormones have JH‐like effects on the follicle cells of Locusta migratoria in vitro
Author(s) -
Davey K.G.,
Gordon D.R.B.
Publication year - 1996
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1996)32:3/4<613::aid-arch32>3.0.co;2-d
Subject(s) - medicine , endocrinology , juvenile hormone , triiodothyronine , hormone , biology , receptor , in vitro , thyronine , ouabain , biochemistry , chemistry , organic chemistry , sodium
Earlier work had shown that JH acts on the membrane of the follicle cell of Locusta migratoria, bringing about a rapid reduction in volume which can be detected in vitro by measuring the increase in optical path difference using quantitative interference microscopy. The juvenoid fenoxycarb, a phenoxyphenyl derivative, is unrelated in structure to the juvenile hormones (which are derivatives of farnesoic acid), but it also caused a reduction in volume of the cells in vitro as measured by an increase in the optical path difference. The vertebrate hormone thyroxine, and thyronine, the non‐iodinated derivative of thyroxine, also phenoxy phenyl compounds, evoked a response like fenoxycarb. The effect of thyroxine was abolished by ouabain, which inhibits Na + /K + ATPase, the effector molecule for JH, and inhibited by ethoxyzolamide which inhibits the binding of JH to a putative membrane receptor. Triiodothyronine, the effective vertebrate hormone, acted at a lower threshold and optimum concentration, and had a greater magnitude of effect than the other compounds tested. These facts suggest that these phenoxyphenyl compounds are JH agonists and that the membrane receptor for JH may resemble a possible membrane receptor for thyroxine. © 1996 Wiley‐Liss, Inc.