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Artificial selection on JHE activity in Gryllus assimilis: Nature of activity differences between lines and effect on JH binding and metabolism
Author(s) -
Zera Anthony J.,
Sall Jeffrey,
Schwartz Robert
Publication year - 1996
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1996)32:3/4<421::aid-arch14>3.0.co;2-e
Subject(s) - hemolymph , biology , enzyme assay , esterase , biochemistry , enzyme
Genetic lines of the cricket Gryllus assimilis selected for elevated vs. decreased activity of hemolymph juvenile hormone esterase (JHE) exhibited a 6.8‐fold difference in enzyme activity by the seventh generation of selection. This documents that hemolymph JHE activity has the capability for rapid evolutionary change. This is the only insect endocrine trait for which such data are currently available. The difference in hemolymph JHE activity between the lines was due, to an equivalent degree, to variation in whole‐cricket enzyme activity and allocation of JHE activity to the hemolymph compartment. No differences in kinetic or thermostability characteristics were observed between JHEs from the high‐ vs. low‐selected lines. This suggests that the variation in JHE activity between the lines resulted from selection of variable regulatory genetic factors affecting the synthesis, degradation, or activation of enzyme activity. JHE and JH‐binding activity in the hemolymph are genetically correlated, possibly due to co‐regulation by the same genetic factors. Finally, in vivo JH degradation was significantly elevated in lines selected for elevated JHE activity. This documents that evolutionary changes in JHE activity alter in vivo JH metabolism. © 1996 Wiley‐Liss, Inc.