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Musca domestica hemolymph ferritin
Author(s) -
Capurro Margareth de L.,
Iughetti Paula,
Ribolla Paulo E.M.,
de Bianchi Antonio G.
Publication year - 1996
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1996)32:2<197::aid-arch4>3.0.co;2-w
Subject(s) - hemolymph , ferritin , musca , midgut , biology , biochemistry , larva , pupa , botany
We describe a method for the purification of ferritin from Musca domestica larval hemolymph. Musca ferritin occurs in hemolymph predominantly as a native protein with molecular weight equal to 550,000 and subunits of 26,000. The average iron content of purified ferritin was determined to be 3,000 ± 600 iron atoms per molecule. The iron contents of ferritin was heterogeneous; both fully iron loaded molecules and apoferritin are probably present in the Musca hemolymph. The anti‐ferritin serum raised in rabbit was able to recognize native ferritin but was not reactive with the protein subunits isolated by SDS‐PAGE. The ferritin concentration in hemolymph attains a maximum of 0.28 mg/ml in the wandering stage larvae, decreasing to 0.13 mg/ml at the middle of pupal stadium. The ferritin contents of midgut and fat bodies were also determined. Fat body ferritin content is greatly reduced when the feeding larva passes into wandering stage. © 1996 Wiley‐Liss, Inc.