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Isolation and characterization of Locusta migratoria accessory gland myotropin I (Lom‐AG‐MT‐I) from the brain of the Colorado potato beetle, Leptinotarsa decemlineata
Author(s) -
Spittaels Kurt,
Vankeerberghen Anne,
Schoofs Liliane,
Proost Paul,
Van Damme Jozef,
De Loof Arnold
Publication year - 1996
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/(sici)1520-6327(1996)31:2<149::aid-arch3>3.0.co;2-v
Subject(s) - leptinotarsa , colorado potato beetle , biology , isolation (microbiology) , larva , botany , zoology , microbiology and biotechnology
A novel myotropic Colorado potato beetle peptide, active in the Locusta oviduct motility assay, was isolated from a methanolic extract of 9,000 brain complexes of adult Leptinotarsa decemlineata by means of HPLC. Its sequence is Gly‐Phe‐Lys‐Asn‐Val‐Ala‐Leu‐Ser‐Thr‐Ala‐Arg‐Gly‐Phe‐NH 2 . This peptide is identical to Lom‐AG‐MT‐I, a myotropin previously isolated from the male accessory glands of Locusta migratoria , using the L. migratoria oviduct motility bioassay as a monitoring system. It strongly stimulated the frequency, amplitude, and tonus of the myogenic oviduct contractions, even at low concentrations. © 1996 Wiley‐Liss, Inc.