Premium
Immobilization of cytochrome P‐450 and electrochemical control of its activity
Author(s) -
Sugihara Nobuhiro,
Ogoma Yoshiro,
Abe Koji,
Kondo Yoshiyuki,
Akaike Toshihiro
Publication year - 1998
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/(sici)1099-1581(199805)9:5<307::aid-pat788>3.0.co;2-1
Subject(s) - hydroxylation , clark electrode , camphor , electrochemistry , electrode , cytochrome , ionic strength , nuclear chemistry , materials science , chemistry , enzyme , organic chemistry , aqueous solution , electrolyte
P‐450 cam (camphor‐induced cytochrome P‐450) was immobilized on an indium tin oxide (ITO) electrode by polypyrrole and its activity was controlled electrochemically. The results showed that P‐450 cam was immobilized on the ITO electrode without denaturing and the amount of P‐450 cam could be easily controlled. When, the electric potential was swept repeatedly between 0.4 and 0 V, the remarkable decrease of oxygen in the reaction mixture solution was observed only in the presence of camphor. In addition, hydroxycamphor was detected only in the same system by means of gas chromatography/mass spectroscopy. These results suggested that immobilized P‐450 cam catalyzed the hydroxylation of camphor by the supply of electron from the electrode. The effect of pH and ionic strength on the activity was examined, and it was found that the high activity expressed at the pH of 6.0 – 7.0 and KCl concentration of 0.1 – 0.2 M . The paper is the first report that P‐450 enzyme activity could be controlled artificially. © 1998 John Wiley & Sons, Ltd.