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In situ observation of protein‐adsorbed stearic acid monolayer by Brewster angle microscopy and fluorescence microscopy
Author(s) -
Tanaka Hideaki,
Akatsuka Takahisa,
Ohe Toru,
Ogoma Yoshiro,
Abe Koji,
Kondo Yoshiyuki
Publication year - 1998
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/(sici)1099-1581(199802)9:2<150::aid-pat743>3.0.co;2-n
Subject(s) - brewster's angle , monolayer , microscopy , materials science , in situ , fluorescence microscope , stearic acid , optical microscope , fluorescence , optics , brewster , nanotechnology , chemistry , scanning electron microscope , composite material , organic chemistry , physics
A fluorescence probe, fluorescein isothiocyanate (FITC), was introduced to proteins, and the morphology of protein‐adsorbed stearic acid monolayer was observed by fluorescence microscopy and Brewster angle microscopy (BAM) in order to analyze images. At a low protein concentration, the surface pressure increased as shown by a sigmoidal curve. A number of stripe patterns in the BAM images increased and the shapes became clear with increasing concentration of proteins. Simultaneously, the size of circular islands also became small, and finally disappeared. These results suggest that the very large stripe patterns in the BAM image show the assembly of both proteins and stearic acid molecules, and small circular islands show only the stearic acid molecules. © 1998 John Wiley & Sons, Ltd.