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Studies on a phenolic resin as a carrier for the immobilization of penicillin acylase
Author(s) -
Ye QingLing,
Li YuanXun
Publication year - 1997
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/(sici)1099-1581(199711)8:11<727::aid-pat709>3.0.co;2-j
Subject(s) - immobilized enzyme , benzylpenicillin , hydroquinone , michaelis–menten kinetics , penicillin , penicillin amidase , chemistry , chromatography , enzyme , nuclear chemistry , organic chemistry , enzyme assay , biochemistry , antibiotics
A gel‐type resin was synthesized through the polycondensation of hydroquinone with formaldehyde in the presence of an acidic solution. This resin is an effective carrier for the immobilization of various enzymes and other proteins. Penicillin acylase was immobilized on this carrier to convert benzylpenicillin to 6‐aminopenicillanic acid, which may be employed in the manufacture of semi synthetic penicillins. Factors that affect the activity of immobilized penicillin acylase, such as temperature, pH and amount of native enzyme, were studied. Under suitable conditions, the activity and activity recovery of the immobilized enzyme was 2000 U/g (dry carrier, p ‐dimethylaminobenzaldehyde method) and 50% respectively. The immobilized penicillin acylase shows a remarkable increase in stability. At 40°C and pH 7.0 the value of kinetic Michaelis–Menten constant of the immobilized enzyme ( K m ) is 1.67×10 −3 mol/l. © 1997 John Wiley & Sons, Ltd.