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Chemical modification of E. coli L ‐asparaginase with polyethylene glycol grafted vinylpyrrolidone–maleic anhydride copolymer
Author(s) -
Qian Guoqiang,
Ma Jianbiao,
Zhou Juyan,
He Binglin,
Wang Daobin
Publication year - 1997
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/(sici)1099-1581(199710)8:10<581::aid-pat689>3.0.co;2-#
Subject(s) - maleic anhydride , copolymer , polyethylene glycol , polymer chemistry , peg ratio , antigenicity , maleic acid , asparaginase , trypsin , ethylene glycol , chemistry , enzyme , nuclear chemistry , biochemistry , organic chemistry , polymer , biology , antigen , finance , economics , lymphoblastic leukemia , leukemia , genetics
A series of polyethylene glycol grafted vinylpyrrolidone–maleic anhydride copolymers [P(VMP)] was synthesized by radical copolymerization of vinylpyrrolidone, maleic anhydride and polyethylene glycol maleic acid monoester. Escherichia coli l ‐asparaginase was chemically modified with these copolymers. The modified l ‐asparaginase exhibited the complete loss of antigenicity towards anti‐asparaginase serum from rabbit. The highest enzyme activity of the modified l ‐asparaginase without antigenicity was retained by 59% of the nonmodified one. The modified enzyme was also more resistant to trypsin in vitro. When tested in vivo, the native l ‐asparaginase was quickly cleared from the plasma of rabbits (half‐life time: t 1/2 =1.2 hr), whereas the modified enzyme showed prolonged clearance from plasma (t 1/2 =53 hr). © 1997 John Wiley & Sons, Ltd.